Coding

Part:BBa_K2272018:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD-HAO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 616
    Illegal XhoI site found at 2180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 222
    Illegal NgoMIV site found at 632
    Illegal NgoMIV site found at 1109
    Illegal NgoMIV site found at 1113
    Illegal NgoMIV site found at 2881
    Illegal NgoMIV site found at 3271
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 536
    Illegal BsaI.rc site found at 584
    Illegal BsaI.rc site found at 1151
    Illegal BsaI.rc site found at 3311


Design Notes

The pBAD promoter and the hydroxylamine oxidoreductase coding sequence was ligated with SCAR.


Source

https://parts.igem.org/Part:BBa_K2272005 https://parts.igem.org/Part:BBa_K2272002

References